Optimized Analysis of MCPD‐ and Glycidyl Esters in Edible Oils and Fats Using Fast Alkaline Transesterification and13C‐Correction for Glycidol Overestimation: Validation Including Interlaboratory Comparison
نویسندگان
چکیده
منابع مشابه
Collaborative Study of an Indirect Enzymatic Method for the Simultaneous Analysis of 3-MCPD, 2-MCPD, and Glycidyl Esters in Edible Oils.
A collaborative study was conducted to evaluate an indirect enzymatic method for the analysis of fatty acid esters of 3-monochloro-1,2-propanediol (3-MCPD), 2-monochloro-1,3-propanediol (2-MCPD), and glycidol (Gly) in edible oils and fats. The method is characterized by the use of Candida rugosa lipase, which hydrolyzes the esters at room temperature in 30 min. Hydrolysis and bromination steps ...
متن کاملOptimization of an Indirect Enzymatic Method for the Simultaneous Analysis of 3-MCPD, 2-MCPD, and Glycidyl Esters in Edible Oils.
We developed a novel, indirect enzymatic method for the analysis of fatty acid esters of 3-monochloro-1,2-propanediol (3-MCPD), 2-monochloro-1,3-propanediol (2-MCPD), and glycidol (Gly) in edible oils and fats. Using this method, the ester analytes were rapidly cleavaged by Candida rugosa lipase at room temperature for 0.5 h. As a result of the simultaneous hydrolysis and bromination steps, 3-M...
متن کاملAn Improved Enzymatic Indirect Method for Simultaneous Determinations of 3-MCPD Esters and Glycidyl Esters in Fish Oils.
The enzymatic indirect method for simultaneous determinations of 3-chloro-1, 2-propanediol fatty acid esters (3-MCPD-Es) and glycidyl fatty acid esters (Gly-Es) make use of lipase from Candida cylindracea (previously referred to as C. rugosa). Because of low substrate specificity of the lipase for esters of polyunsaturated fatty acids (PUFA), such as docosahexaenoic acid (DHA) and eicosapentaen...
متن کاملNovel fast analytical method for indirect determination of MCPD fatty acid esters in edible oils and fats based on simultaneous extraction and derivatization
A novel method for indirect determination of MCPD esters levels in lipid samples has been developed. The method is based on combination of extraction and derivatization in the same sample preparation step. It is achieved by the application of diethyl ether as extraction solvent for isolation of analytes released from esterified forms from the water phase and as dilution solvent for solid PBA - ...
متن کاملChallenges in Analysing Glycidyl Fatty Acid Esters in Edilble Oils and Fats - Poster vom 18. September 2011
glycidol undergoing 100 % transformation into 3-MCPD (reference value) (NaCl) [TF=0.67] GE stearate (NaCl) [TF=0.85] GE linolenate (NaCl) [TF=0.67] GE oleate (NaCl) [TF=0.71] glycidol undergoing 100 % transformation into 3-MBPD (reference value) (NaBr) [TF=0.48] GE stearate (NaBr) [TF=0.51] GE linolenate (NaBr) [TF=0.31] Linear (GE linolenate (NaBr) [TF=0.31]) Challenges in Analysing Glycidyl F...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Lipid Science and Technology
سال: 2019
ISSN: 1438-7697,1438-9312
DOI: 10.1002/ejlt.201800395